polymerase chain reaction master mix tube Search Results


mycbov dtec-qpcr master mix  (Genetic PCR Solutions)
90
Genetic PCR Solutions mycbov dtec-qpcr master mix
Mycbov Dtec Qpcr Master Mix, supplied by Genetic PCR Solutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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taqman gene expression master mix  (Thermo Fisher)
99
Thermo Fisher taqman gene expression master mix
Taqman Gene Expression Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard pcr preps dna purification system  (Promega)
90
Promega wizard pcr preps dna purification system
Wizard Pcr Preps Dna Purification System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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nebnext ffpe dna repair mix  (New England Biolabs)
98
New England Biolabs nebnext ffpe dna repair mix
Nebnext Ffpe Dna Repair Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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platinum superfi dna polymerase master mix  (Thermo Fisher)
99
Thermo Fisher platinum superfi dna polymerase master mix
Platinum Superfi Dna Polymerase Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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revertraace qpcr rt master mix  (Toyobo)
99
Toyobo revertraace qpcr rt master mix
(A) Summary of the micrografting and transfer experiments. (B, C) Nitrate concentrations in shoots (B) and roots (C) 0, 3, or 5 days after nitrogen removal. (D, E) Total protein concentrations in shoots (D) and roots (E) 5 days after nitrogen removal. (B–E) Two grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (F, G) Total nitrogen concentrations in shoots (F) and roots (G) 5 days after nitrogen removal. Sampling was performed three times independently. In this sampling, 7, 6, and 5 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (B–G) Different lowercase letters indicate significant differences, as determined via Tukey– Kramer test ( P < 0.05). (H) Transcript levels of IPT3 in shoots 5 days after nitrogen removal, as determined using <t>RT-qPCR.</t> Sampling was done five times independently. In this sampling, 9, 6, 7, 7, and 8 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 5). (I) Nitrate concentrations in shoots 5 days after nitrogen removal. (J) Transcript levels of GFP in shoots 5 days after nitrogen removal, as determined via RT-qPCR. (I, J) One biological replicate consisted of one grafted plant. Data are presented as mean ± SD (n = 3). (H–J) Statistical significance was assessed using unpaired two-tailed Welch’s t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). (K) Fluorescence images of true leaves 5 days after nitrogen removal. Green and red colors correspond to GFP fluorescence and autofluorescence, respectively. Scale bars represent 500 μm. (B–K) In graph legends, the grafted plants are indicated as “scion line name/rootstock line name.” ns, not significant; FW, fresh weight; DW, dry weight.
Revertraace Qpcr Rt Master Mix, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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gdna remover  (Toyobo)
99
Toyobo gdna remover
(A) Summary of the micrografting and transfer experiments. (B, C) Nitrate concentrations in shoots (B) and roots (C) 0, 3, or 5 days after nitrogen removal. (D, E) Total protein concentrations in shoots (D) and roots (E) 5 days after nitrogen removal. (B–E) Two grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (F, G) Total nitrogen concentrations in shoots (F) and roots (G) 5 days after nitrogen removal. Sampling was performed three times independently. In this sampling, 7, 6, and 5 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (B–G) Different lowercase letters indicate significant differences, as determined via Tukey– Kramer test ( P < 0.05). (H) Transcript levels of IPT3 in shoots 5 days after nitrogen removal, as determined using <t>RT-qPCR.</t> Sampling was done five times independently. In this sampling, 9, 6, 7, 7, and 8 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 5). (I) Nitrate concentrations in shoots 5 days after nitrogen removal. (J) Transcript levels of GFP in shoots 5 days after nitrogen removal, as determined via RT-qPCR. (I, J) One biological replicate consisted of one grafted plant. Data are presented as mean ± SD (n = 3). (H–J) Statistical significance was assessed using unpaired two-tailed Welch’s t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). (K) Fluorescence images of true leaves 5 days after nitrogen removal. Green and red colors correspond to GFP fluorescence and autofluorescence, respectively. Scale bars represent 500 μm. (B–K) In graph legends, the grafted plants are indicated as “scion line name/rootstock line name.” ns, not significant; FW, fresh weight; DW, dry weight.
Gdna Remover, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gibson assembly  (New England Biolabs)
99
New England Biolabs gibson assembly
(A) Summary of the micrografting and transfer experiments. (B, C) Nitrate concentrations in shoots (B) and roots (C) 0, 3, or 5 days after nitrogen removal. (D, E) Total protein concentrations in shoots (D) and roots (E) 5 days after nitrogen removal. (B–E) Two grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (F, G) Total nitrogen concentrations in shoots (F) and roots (G) 5 days after nitrogen removal. Sampling was performed three times independently. In this sampling, 7, 6, and 5 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (B–G) Different lowercase letters indicate significant differences, as determined via Tukey– Kramer test ( P < 0.05). (H) Transcript levels of IPT3 in shoots 5 days after nitrogen removal, as determined using <t>RT-qPCR.</t> Sampling was done five times independently. In this sampling, 9, 6, 7, 7, and 8 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 5). (I) Nitrate concentrations in shoots 5 days after nitrogen removal. (J) Transcript levels of GFP in shoots 5 days after nitrogen removal, as determined via RT-qPCR. (I, J) One biological replicate consisted of one grafted plant. Data are presented as mean ± SD (n = 3). (H–J) Statistical significance was assessed using unpaired two-tailed Welch’s t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). (K) Fluorescence images of true leaves 5 days after nitrogen removal. Green and red colors correspond to GFP fluorescence and autofluorescence, respectively. Scale bars represent 500 μm. (B–K) In graph legends, the grafted plants are indicated as “scion line name/rootstock line name.” ns, not significant; FW, fresh weight; DW, dry weight.
Gibson Assembly, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymerase+chain+reaction+master+mix+tube/Gibson+Assembly+Master+Mix/pm38448444-197-32-34
Average 99 stars, based on 1 article reviews
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1x onetaq dna polymerase quick load master mix  (New England Biolabs)
96
New England Biolabs 1x onetaq dna polymerase quick load master mix
(A) Summary of the micrografting and transfer experiments. (B, C) Nitrate concentrations in shoots (B) and roots (C) 0, 3, or 5 days after nitrogen removal. (D, E) Total protein concentrations in shoots (D) and roots (E) 5 days after nitrogen removal. (B–E) Two grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (F, G) Total nitrogen concentrations in shoots (F) and roots (G) 5 days after nitrogen removal. Sampling was performed three times independently. In this sampling, 7, 6, and 5 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (B–G) Different lowercase letters indicate significant differences, as determined via Tukey– Kramer test ( P < 0.05). (H) Transcript levels of IPT3 in shoots 5 days after nitrogen removal, as determined using <t>RT-qPCR.</t> Sampling was done five times independently. In this sampling, 9, 6, 7, 7, and 8 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 5). (I) Nitrate concentrations in shoots 5 days after nitrogen removal. (J) Transcript levels of GFP in shoots 5 days after nitrogen removal, as determined via RT-qPCR. (I, J) One biological replicate consisted of one grafted plant. Data are presented as mean ± SD (n = 3). (H–J) Statistical significance was assessed using unpaired two-tailed Welch’s t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). (K) Fluorescence images of true leaves 5 days after nitrogen removal. Green and red colors correspond to GFP fluorescence and autofluorescence, respectively. Scale bars represent 500 μm. (B–K) In graph legends, the grafted plants are indicated as “scion line name/rootstock line name.” ns, not significant; FW, fresh weight; DW, dry weight.
1x Onetaq Dna Polymerase Quick Load Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gibson assembly master mix  (New England Biolabs)
99
New England Biolabs gibson assembly master mix
(A) Summary of the micrografting and transfer experiments. (B, C) Nitrate concentrations in shoots (B) and roots (C) 0, 3, or 5 days after nitrogen removal. (D, E) Total protein concentrations in shoots (D) and roots (E) 5 days after nitrogen removal. (B–E) Two grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (F, G) Total nitrogen concentrations in shoots (F) and roots (G) 5 days after nitrogen removal. Sampling was performed three times independently. In this sampling, 7, 6, and 5 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (B–G) Different lowercase letters indicate significant differences, as determined via Tukey– Kramer test ( P < 0.05). (H) Transcript levels of IPT3 in shoots 5 days after nitrogen removal, as determined using <t>RT-qPCR.</t> Sampling was done five times independently. In this sampling, 9, 6, 7, 7, and 8 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 5). (I) Nitrate concentrations in shoots 5 days after nitrogen removal. (J) Transcript levels of GFP in shoots 5 days after nitrogen removal, as determined via RT-qPCR. (I, J) One biological replicate consisted of one grafted plant. Data are presented as mean ± SD (n = 3). (H–J) Statistical significance was assessed using unpaired two-tailed Welch’s t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). (K) Fluorescence images of true leaves 5 days after nitrogen removal. Green and red colors correspond to GFP fluorescence and autofluorescence, respectively. Scale bars represent 500 μm. (B–K) In graph legends, the grafted plants are indicated as “scion line name/rootstock line name.” ns, not significant; FW, fresh weight; DW, dry weight.
Gibson Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymerase+chain+reaction+master+mix+tube/Gibson+Assembly+Master+Mix/pmc10949428-383-23-27
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nebuilder hifi dna assembly master mix  (New England Biolabs)
99
New England Biolabs nebuilder hifi dna assembly master mix
(A) Summary of the micrografting and transfer experiments. (B, C) Nitrate concentrations in shoots (B) and roots (C) 0, 3, or 5 days after nitrogen removal. (D, E) Total protein concentrations in shoots (D) and roots (E) 5 days after nitrogen removal. (B–E) Two grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (F, G) Total nitrogen concentrations in shoots (F) and roots (G) 5 days after nitrogen removal. Sampling was performed three times independently. In this sampling, 7, 6, and 5 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (B–G) Different lowercase letters indicate significant differences, as determined via Tukey– Kramer test ( P < 0.05). (H) Transcript levels of IPT3 in shoots 5 days after nitrogen removal, as determined using <t>RT-qPCR.</t> Sampling was done five times independently. In this sampling, 9, 6, 7, 7, and 8 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 5). (I) Nitrate concentrations in shoots 5 days after nitrogen removal. (J) Transcript levels of GFP in shoots 5 days after nitrogen removal, as determined via RT-qPCR. (I, J) One biological replicate consisted of one grafted plant. Data are presented as mean ± SD (n = 3). (H–J) Statistical significance was assessed using unpaired two-tailed Welch’s t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). (K) Fluorescence images of true leaves 5 days after nitrogen removal. Green and red colors correspond to GFP fluorescence and autofluorescence, respectively. Scale bars represent 500 μm. (B–K) In graph legends, the grafted plants are indicated as “scion line name/rootstock line name.” ns, not significant; FW, fresh weight; DW, dry weight.
Nebuilder Hifi Dna Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tubes dna polymerase i  (New England Biolabs)
98
New England Biolabs tubes dna polymerase i
(A) Summary of the micrografting and transfer experiments. (B, C) Nitrate concentrations in shoots (B) and roots (C) 0, 3, or 5 days after nitrogen removal. (D, E) Total protein concentrations in shoots (D) and roots (E) 5 days after nitrogen removal. (B–E) Two grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (F, G) Total nitrogen concentrations in shoots (F) and roots (G) 5 days after nitrogen removal. Sampling was performed three times independently. In this sampling, 7, 6, and 5 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (B–G) Different lowercase letters indicate significant differences, as determined via Tukey– Kramer test ( P < 0.05). (H) Transcript levels of IPT3 in shoots 5 days after nitrogen removal, as determined using <t>RT-qPCR.</t> Sampling was done five times independently. In this sampling, 9, 6, 7, 7, and 8 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 5). (I) Nitrate concentrations in shoots 5 days after nitrogen removal. (J) Transcript levels of GFP in shoots 5 days after nitrogen removal, as determined via RT-qPCR. (I, J) One biological replicate consisted of one grafted plant. Data are presented as mean ± SD (n = 3). (H–J) Statistical significance was assessed using unpaired two-tailed Welch’s t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). (K) Fluorescence images of true leaves 5 days after nitrogen removal. Green and red colors correspond to GFP fluorescence and autofluorescence, respectively. Scale bars represent 500 μm. (B–K) In graph legends, the grafted plants are indicated as “scion line name/rootstock line name.” ns, not significant; FW, fresh weight; DW, dry weight.
Tubes Dna Polymerase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Summary of the micrografting and transfer experiments. (B, C) Nitrate concentrations in shoots (B) and roots (C) 0, 3, or 5 days after nitrogen removal. (D, E) Total protein concentrations in shoots (D) and roots (E) 5 days after nitrogen removal. (B–E) Two grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (F, G) Total nitrogen concentrations in shoots (F) and roots (G) 5 days after nitrogen removal. Sampling was performed three times independently. In this sampling, 7, 6, and 5 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (B–G) Different lowercase letters indicate significant differences, as determined via Tukey– Kramer test ( P < 0.05). (H) Transcript levels of IPT3 in shoots 5 days after nitrogen removal, as determined using RT-qPCR. Sampling was done five times independently. In this sampling, 9, 6, 7, 7, and 8 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 5). (I) Nitrate concentrations in shoots 5 days after nitrogen removal. (J) Transcript levels of GFP in shoots 5 days after nitrogen removal, as determined via RT-qPCR. (I, J) One biological replicate consisted of one grafted plant. Data are presented as mean ± SD (n = 3). (H–J) Statistical significance was assessed using unpaired two-tailed Welch’s t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). (K) Fluorescence images of true leaves 5 days after nitrogen removal. Green and red colors correspond to GFP fluorescence and autofluorescence, respectively. Scale bars represent 500 μm. (B–K) In graph legends, the grafted plants are indicated as “scion line name/rootstock line name.” ns, not significant; FW, fresh weight; DW, dry weight.

Journal: bioRxiv

Article Title: Shoot nitrate status regulates Arabidopsis shoot growth and systemic transcriptional responses via shoot adenylate isopentenyltransferase 3

doi: 10.1101/2024.12.26.630360

Figure Lengend Snippet: (A) Summary of the micrografting and transfer experiments. (B, C) Nitrate concentrations in shoots (B) and roots (C) 0, 3, or 5 days after nitrogen removal. (D, E) Total protein concentrations in shoots (D) and roots (E) 5 days after nitrogen removal. (B–E) Two grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (F, G) Total nitrogen concentrations in shoots (F) and roots (G) 5 days after nitrogen removal. Sampling was performed three times independently. In this sampling, 7, 6, and 5 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 3). (B–G) Different lowercase letters indicate significant differences, as determined via Tukey– Kramer test ( P < 0.05). (H) Transcript levels of IPT3 in shoots 5 days after nitrogen removal, as determined using RT-qPCR. Sampling was done five times independently. In this sampling, 9, 6, 7, 7, and 8 grafted plants were pooled as one biological replicate. Data are presented as mean ± SD (n = 5). (I) Nitrate concentrations in shoots 5 days after nitrogen removal. (J) Transcript levels of GFP in shoots 5 days after nitrogen removal, as determined via RT-qPCR. (I, J) One biological replicate consisted of one grafted plant. Data are presented as mean ± SD (n = 3). (H–J) Statistical significance was assessed using unpaired two-tailed Welch’s t -test (* P < 0.05; ** P < 0.01; *** P < 0.001). (K) Fluorescence images of true leaves 5 days after nitrogen removal. Green and red colors correspond to GFP fluorescence and autofluorescence, respectively. Scale bars represent 500 μm. (B–K) In graph legends, the grafted plants are indicated as “scion line name/rootstock line name.” ns, not significant; FW, fresh weight; DW, dry weight.

Article Snippet: Reverse transcription (RT) was performed using a ReverTraAce qPCR RT Master Mix with gDNA Remover (Toyobo, Osaka Japan).

Techniques: Sampling, Quantitative RT-PCR, Two Tailed Test, Fluorescence